Diagnosis of leukemias by Southern blotting.

Diagnosis of cutaneous tuberculosis in biopsy specimens by PCR and southern blotting.

AIMS To evaluate the use of a gene amplification and hybridisation method for detecting mycobacterial nucleic acid as a possible diagnostic method for cutaneous tuberculosis infection. METHODS Biopsy specimens from 20 patients with various skin conditions of possible tuberculous aetiology were studied. Six patients had ulcerative nodules, seven lupiform lesions, two non-necrotic granulomas, o...

Southern Blotting and Related DNA Detection Techniques

Southern blotting is one of the central techniques in molecular biology. First devised by E.M. Southern (1975), Southern blotting results in transfer of DNA molecules, usually restriction fragments, from an electrophoresis gel to a nitrocellulose or nylon sheet (referred to as a ‘membrane’), in such a way that the DNA banding pattern present in the gel is reproduced on the membrane. During tran...

Cluster of Differentiation 45: An Adjunct to Flowcytometric Diagnosis of Leukemias

Background: This Cross sectional comparative study was performed to compare the mean fluorescence intensity of cluster of differentiation 45 between healthy individuals and patients with acute lymphoblastic leukemia. Patients and methods: Thirty three healthy individuals (mean age 11 years) and 41 patients with B cell acute lymphoblastic leukemia (mean age 7 years) were enrolled in the study...

western blotting method for diagnosis of hydatid cyst antigens

Evaluation of Echinococcus granulosus coproantigens by Dot-blotting in dogs

Definitive hosts of the Echinococcus granulosus (E. granulosus ) parasite are carnivores such as dogs,wolves and foxes. Detection of this parasite through faecal examination is not possible. In this study, dotblotting test for E. granulosus -specific coproantigens has been evaluated in dog. Three 2–3-month-old puppies were treated with piperazine and then faecal samples were collected as pre-in...